The Utility of the Deuterated Spin Label in the Spin Immunoassay of Cortisol
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概要
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A deuterated spin-labeled derivative of cortisol (F3-SLD) was prepared by coupling cortisol-3-(O-carboxymethyl) oxime with 4-amino-1-oxyl-2,2,6,6-tetramethylpiperidine-d_<17> and used to improve the sensitivity of spin immunoassay (SIA) of cortisol. The linewidth of the high-field peak of F3-SLD was 1.4G at the modulation amplitude (H_m) of 1.6G, and that of the undeuterated spin-label was 1.9G at H_m=2.0G. When 200 μl of cortisol standards was used for the assay, the minimum detectable concentration of cortisol was reduced from 10 ng/200 μl to 2.5 ng/200 μl by the use of F3-SLD. Although the increase in the sensitivity of SIA is not yet sufficient, the use of deuterated spin-labels is considered to be useful for enhancing SIA sensitivity.
- 公益社団法人日本薬学会の論文
- 1983-09-25
著者
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細川 幹夫
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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佐用 博照
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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細川 幹夫
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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