大麻幻覚成分Δ^9-Tetrahydrocannabinolのヒト尿中主代謝物Δ^9-Tetrahydrocannabinol-11-oic AcidのGC-MSによる定性・定量

概要

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The GC-MS and TLC methods for the determination and identification of Δ^9-tetrahydrocannabinol-11-oic acid, a major urinary metabolite of Δ^9-tetrahydrocannabinol which is one of main constituents of marihuana, were established. Δ^9-Tetrahydrocannabinol-11-oic acid was detected on TLC by using Fast Blue BB salt as a color reagent. The metabolite was determined as trimethylsilyl derivative of its methyl ester by using electron impact ionization GC-MS and 5'-nor-Δ^8-tetrahydrocannabinol-4'-oic acid as an internal standard. The assay provides excellent sensitivity, reproducibility and specificity with detection limit of 1 ng/ml human urine.
社団法人日本薬学会の論文
1990-04-28