チアベンダゾール(TBZ)経口投与雌雄マウスの肝ミクロゾーム薬物代謝酵素活性

概要

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Enzyme activities of liver microsomal cytochromes P-450 induced by thiabendazole (TBZ) which has been used as a food additive were investigated in 5 and 11 week-old mice (male and female). TBZ was suspended in olive oil and mice were given orally at 350,700 or 1000 mg/kg/d for 3 d. Cytochromes P-450 were purified from mice liver microsomes by EAH Sepharose 4B and DEAE Sephadex A-50 chromatography. The catalytic activities of mice liver microsomes and purified cytochromes P-450 fractions on TBZ, aniline, aminopyrine and biphenyl were examined. TBZ hydroxylase, aniline hydroxylase and biphenyl 4-hydroxylase of liver microsomes were induced by TBZ in mice, but aminopyrine N-demethylase was not induced. TBZ hydroxylase activity was higher in female than male in 11 week-old mice. In a reconstituted system of cytochromes P-450,the fractions from TBZ-treated mice showed higher activities of TBZ and aniline hydroxylases than the corresponding fractions from untreated mice.
社団法人日本薬学会の論文
1995-06-30