3-メチル-2-ベンゾチアゾリノンヒドラゾンによる糖類の比色定量

概要

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The reaction of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with sugars resulted in the development of green to blue coloration, which has an absorption maximum at about 635 mμ. Therefore, the colorimetric determination of sugars using MBTH was examined. To 1 ml of sample, 1 ml of 0.4% MBTH solution and 2 ml of 0.05 N NaOH are added, and the mixture is heated in a boiling water bath for 10 minutes, then cooled under cold tap water to room temperature. Five ml of 1% ferric chloride solution are added. Following about 10 minute waiting period, the mixture is extracted with n-butyl alcohol, then the absorbance at 635 mμ is mesured. The concentration range of glucose determinable by this method is 5-70 μg/ml, and the determined value agree with Somogyi-Nelson's method within the largest deviation of ± 3%.
社団法人日本薬学会の論文
1969-08-30