Accumulation and Subcellular Localization of Cesium in Mycelia of Streptomyces lividans and a Cs Tolerant Strain, Streptomyces sp. TOHO-2
スポンサーリンク
概要
- 論文の詳細を見る
Cs uptake in Streptomyces lividans TK24 and Streptomyces sp. TOHO-2,as representatives of actinomycetes, was investigated. Growth of S. lividans TK24 was inhibited above 50 mM CsCl in yeast extract-malt extract agar, while that of Streptomyces sp. TOHO-2,which was isolated from soil as a Cs tolerant strain, was inhibited above 200 mM CsCl. The amount of Cs incorporated into the mycelia of these strains increased with the concentration of Cs in the medium; S. lividans TK24,grown in the presence of 25 mM Cs, accumulated 61.5 mgCs/g dry weight of mycelia, and Streptomyces sp. TOHO-2,grown in the presence of 150 mM Cs, accumulated 142.2 mgCs/g dry weight of mycelia. Scanning electron microscopy (SEM) of the substrate mycelia grown on the YM agar plate containing CsCl showed white spots with a similar distribution of mycelia. Elemental analysis using SEM-energy dispersive X-ray analysis showed that there were larger amounts of Cs, P and O in the brilliant spots than in other regions of the cell. Streptomyces lividans TK24 and Streptomyces sp. TOHO-2 could grow even in the presence of high concentrations of Cs, and accumulated high concentration of Cs in the cells.
- 社団法人日本薬学会の論文
- 2000-08-01
著者
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杉山 英男
国立保健医療科学院
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ICHIKAWA Takayuki
Department of Materials Science and Engineering, Kyoto University
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Takami H
Subaru Telescope Naoj
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Sugiyama Hideo
National Institute of Public Health
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Terada Hiroshi
National Institute of Public Health
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Morita Yohoji
Application Center, Horiba Co., Ltd.
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Kato Fumio
Department of Microbiology, School of Pharmaceutical Sciences, Toho University
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Kuwahara Chikako
Division of Enviromental Health, Kanagawa Prefectural Public Health Laboratory
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Oosone Ayako
Department of Microbiology, School of Pharmaceutical Sciences, Toho University
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Terada H
National Institute Of Public Health
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杉山 英男
Dep. Of Environmental Health National Inst. Of Public Health
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Kato Fumio
産業医科大学 放射線衛生
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Fumio Kato
Department Of Microbiology School Of Pharmaceutical Sciences Toho University
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Kato Fumio
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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Sugiyama Hidetoshi
Kanagawa Environmental Research Center
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Ichikawa Takayuki
Department Of Materials Science And Engineering Kyoto University
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Ichikawa Takayuki
Department Of Microbiology School Of Pharmaceutical Sciences Toho University
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Morita Yohoji
Application Center Horiba Co. Ltd.
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Oosone Ayako
Department Of Microbiology School Of Pharmaceutical Sciences Toho University
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Sugiyama Hideo
Dep. Of Environmental Health National Inst. Of Public Health
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Hisashi Shibata
Yamanashi Forestry And Forest Products Research Institute
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Kuwahara Chikako
Division Of Enviromental Health Kanagawa Prefectural Public Health Laboratory
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Kato Fumio
Department Of Agricultural Chemistry Saga University
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