Purification and Characterization of the Antioxidative Substance Produced by Aspergillus sojae K
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概要
- 論文の詳細を見る
An antioxidative substance produced by Aspergillus sojae K is absolutely soluble in water and strongly inhibits autoxidation of Na-ascorbate. The substance, produced extracellularly in the culture fluid by the mold, was purified by ion exchange chromatography, gel filtration, and HPLC. The substance was purified 34-fold with an activity recovery of 38% from culture fluid. Purity of the substance was confirmed with a single peak through HPLC using an analytical column as well as a single spot on TLC. The purified substance consists of equimolar aspartic acid and glycine, indicating that the substance is a peptide. From mass spectral analysis the molecular weight was 710, but the precise sequence of the amino acids is not clear. The substance is stable at 70℃, but about 80% of the activity was lost at 80℃ after 60 min. Besides, the substance is completely stable at pH 3-14. This substance efficiently suppressed the oxidation of fish oil.
- 社団法人日本農芸化学会の論文
- 1993-06-23
著者
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Murata Akira
Department Of Cardiology Toyohashi Heart Center
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Kato Fumio
Department of Microbiology, School of Pharmaceutical Sciences, Toho University
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Harun-or-rashid Md.
Department Of Biochemistry Bangladesh Agricultural University
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KONDO Michio
Department of Chemistry, Faculty of Science and Engineering, Saga University
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Kato Fumio
Dept. Radiat. Biol. & Health Sch. Med. Univ. Of Occup. & Environm. Health
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Kato Fumio
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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Kato Fumio
Department Of Applied Biological Science Saga University
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Murata Akira
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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Murata Akira
Department Of Mechanical Systems Engineering Tokyo University Of A&t
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Murata A
Department Of Mechanical Systems Engineering Tokyo University Of Agriculture And Technology
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Kato F
Department Of Applied Biological Science Faculty Of Agriculture Saga University
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Kato Fumio
Department Of Agricultural Chemistry Saga University
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Kondo Michio
Department Of Chemistry Saga University
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Kondo Michio
Department Of Chemistry Faculty Of Science And Ehgineering Saga University
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Murata Akira
Department Of Agricultural Chemistry Saga University
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