Direct Separation and Determination of Synephrine Enantiomers by High-Performance Liquid Chromatography with Electrochemical Detection

概要

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High-performance liquid chromatography (HPLC) with electrochemical detection using a chiral ligand-exchange column (Sumichiral OA-5000) and a mobile aqueous phase containing 1 mM copper(II) acetate and 20 mM ammonium acetate (pH 6.4) was applied to the direct separation and determination of synephrine enantiomers. The calibration curve for each enantiomer showed good linearity (r=0.999) over a concentration range of 0.2-100 μM with a detection limit of 0.2 μM (signal-to-noise ratio (S/N)=3). As far as reproducibility was concerned, the relative standard deviation (R.S.D.) was 2.6% at 20 μM d-synephrine and 2.4% at 20μM-l-synephrine. The degradation and optical isomerization of l-synephrine in Citrus unshiu were caused by ultraviolet (UV) light or heat. The synephrine enantiomers in citrus plants, crude drugs and Chinese medicines were determined by the present method.
公益社団法人日本薬学会の論文
1995-07-15