ルミノール化学発光法を用いるコリン含有リン脂質のフローインジェクション分析法

概要

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A sensitive flow injection analysis using luminol/peroxidase chemiluminescence was developed for the determination of choline-containing phospholipids in serum. Flow injection manifold was composed of two channel system with an enzyme column, in which phospholipase D was immobilized together with choline oxidase. The serum sample (5 μl) was pretreated by Extrelut column (diatomite column) extraction with chloroform-methanol (95 : 5). The extract (20μl) was injected into a sample carrier at 38℃ and passed through the enzyme column, which converted phospholipid to choline and subsequently to hydrogen peroxide. Produced hydrogen peroxide was monitored by measuring the chemiluminescence intensity of luminol/peroxidase system at 5℃. The response was linear against the amount of phospholipids ranging from 2 to 2000 pmol/test, and the relative standard deviation was less than 2%. In the determination of phospholipids in the serum, a correlation coefficient (r) between 4-aminoantipyrine/phenol and the proposed methods was found to be 0.983 (Y=1.035X-6.2). The throughput rate was 15 samples/h.
社団法人日本薬学会の論文
1992-01-25