Stimulatory Action of Sodium Dodecyl Sulfate (SDS) on the Lecithin-Cholesterol Acyltransferase Reaction in Human Plasma

概要

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The esterification of cholesterol in sonicated dispersions with lecithin-cholesterol molar ratios of 1.5 and 3.7 by human plasma was specifically stimulated by the addition of 5×10^<-4>M sodium dodecyl sulfate (SDS) but not by the addition of cetyltrimethylammonium chloride (CTAC) or Tween-20. On the other hand, when residual protein fraction (d>1.210g/cm^3) of human plasma instead of human plasma was used as the enzyme source, the esterification of cholesterol in the dispersion with a molar ratio of 1.5 was also stimulated at 5×10^<-4>M SDS while that in the dispersion with a molar ratio of 3.7 was not stimulated. However, upon addition of high density lipoprotein (HDL) (1.063<d<1.210g/cm^3), the esterification of cholesterol in the dispersion with a molar ratio of 3.7 (3.9) as well as that with a ratio of 1.5 (1.1) was also stimulated. Similarly, upon addition of 1×10^<-3>M mercaptoethanol, the stimulatory action of SDS on the esterification of cholesterol in the dispersion with a molar ratio of 3.7 (3.9) was observed even in the absence of exogenous HDL. The stimulatory action of SDS on the acyltransferase reaction in human plasma is discussed on the basis of the above results.
社団法人日本薬学会の論文
1984-04-25