培養ヒト膀胱癌細胞の増殖動態の解明 : とくに蛋白合成とcell viabilityとの関係について

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Cell population kinetics in the established cell line of human bladder carcinoma, T24 cells, was investigated in terms of DNA and protein synthesis during cell growth. The growth fraction was estimated to be higher than 96% in the log phase and then rapidly reduced to a final level of 2% in the stationary phase. On the other hand, the labeling index with ^<14>C-leucine was continuously higher than 98% through the cell growth, indicating that both the proliferating and resting cells keep the protein synthesis. ^<14>C-leucine incorporation into the log phase culture as well as into the stationary phase culture was also found to correlate well with the viable cell number. Experiments employing a cytotoxic agent again revealed that ^<14>C-leucine incorporation satisfactorily reflected the cell viability. The results of the present work suggest that ^<14>C-leucine incorporation can be used as a reliable measure for the in vitro assay system.
社団法人日本泌尿器科学会の論文