アミノ酸酵素電極を用いたアミノ酸の光学純度測定用フローインジェクション分析システム

概要

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A simple and rapid method is presented for the measurement of the optical purities of amino acids, using the FIA system with L- and D-amino acid enzyme-electrodes which give specific response for L- and D-amino acids respectively. The enzyme electrode is made up of cross-linking L-amino acid oxidase or D-amino acid oxidase with bovine serum albumin using glutaraldehyde on a flow-through platinum electrode silanized with (3-aminopropyl) triethoxysilane. Both enzyme electrodes are based on the amperometric detection of hydrogen peroxide generated by the enzymatic reactions. The proposed method is highsensitive for some amino acids (methionine, tyrosine, leucine and phenylalanine) and is useful for the specific detection of L- and D-isomers of amino acids. The peak current is linearly related to the concentration of L- or D-amino acids in the range 10^<-5>〜2×10^<-3> M, for injections of 20-μl samples. The measurements can be performed at a rate of 100〜120 samples h^<-1> with satisfactory precision (1.1〜2.8% R. S. D.). The enzyme electrodes retained 82〜91% of their original activities after repetitive use for two months.
社団法人日本分析化学会の論文
1988-07-05