亜硫酸及びリン酸イオン酵素センサーの開発とイオン特異性

概要

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A sulfite ion enzyme sensor was prepared by cross-linking sulfite oxidase with bovine serum albumin using glutaraldehyde on the flat end of a platinum disk electrode silanized with 3-aminopropyltrimethoxysilane. Similarly, a phosphate ion enzyme sensor was made by coimmobilizing purine nucleoside phosphorylase and xanthine oxidase on the flat end of a glassy carbon disk electrode. A sulfite sensor was based on the amperometric detection(0.6V vs. Ag/AgCl) of hydrogen peroxide produced by an enzymatic reaction; the current response was linearly related to the sulfite concentration over the range of 5×10^<-7>〜5×10^<-4>M. This sensor was highly selective for sulfite, except for a small response to nitrite, thiocyanate and thiosulfate. A phosphate sensor gave a response when inosine (5mM) was added to a 0.1M borate buffer solution (pH 7.5) containing phosphate. This sensor was based on the amperometric detection (0.5V vs. Ag/AgCl) of uric acid produced by two successive enzymatic reactions. The current response was linearly related to the phosphate concentration over a range of 5×10^<-7>〜2×10^<-5>M at 37±0.2℃. This sensor was essentially specific for phosphate.
社団法人日本分析化学会の論文
1993-06-05