固定化酵素を利用したフローインジェクション-電流測定法による血清中のエタノールの定量

概要

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A flow injection system for enzymatic determination of ethanol in serums is described. A phosphate buffer (0.1 M, pH 8.0) containing 1 mM potassium hexacyanoferrate (II) served as the carrier solution and was pumped at a constant flow rate of 1.4mlmin^<-1>. The system includes an amperometric peroxidase electrode to measure hexacyanoferrate (III) converted from hexacyanoferrate (II) by hydrogen peroxide, which is gen erated by injecting 10-μl sample into an immobilized alcohol oxidase reactor. The peroxidase electrode operates at 0.0 V vs. Ag/AgCl. The peak current was linearly proportional to the ethanol concentration in the range of 0.02〜10mM ; 120〜140 samples per h could be processed with satisfactory precision (1〜2% R. S. D.).
社団法人日本分析化学会の論文
1985-08-05