菌株保存に関する研究 (第2報) : 有胞子酵母の流動パラフィンによる保存 (その2)

概要

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Previously we reported that about seventy per cent of the strains of sporogenous yeasts preserved under mineral oil at room temperature were maintained sucessfully for over six years. From the results of the recultivation from these stock cultures on malt agar slant, however, it was supposed that the survivors of certain strains must be very rare. Therefore the following ten strains of these cultures were selected from the view-point of their survival ratios or taxonomical interests, and each of the ten isolates from these strains were compared with the subculturing strains in regard to sporulation and several physiological properties. The selected strains were labeled as Schizos. pombe(=S. mellacci), S. cerevisiae(=S. sake and Zygos. priorianus), S. lactis, S. rouxii var. polymorphus (=Zygos. barkeri, ), Z. naniwaensis, Z. nukamiso, Z. sake, Hansenula minuta and Saccharomycodes ludwigii. Consequently, some quantitative differences of a few strains, between these isolates and their subculturing strains, were found in sporulation, in the fermentatin of sucrose, maltose or raffinose and in the assimilation of galactose or maltose. However, qualitative differences were not detected. From these results, it is believed that this preserving method is more practical than other now used.
社団法人日本生物工学会の論文
1963-06-15