菌株保存に関する研究 (第1報) : 有胞子酵母の流動パラフィンによる保存(その1)

概要

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The longevity of 139 strains of ascosporogenous yeasts, including 9 genera, 39 species and 4 varieties, stored under mineral oil were tested. These yeasts were cultivated on malt agar slants until maximum growth was attained.The slants were then overlaid with sterile paraffin oil, and stored at room temperature that changed from 5℃ in winter to 34℃ in summer.The viability of these cultures were tested after 27,38,50,61 and 75 months, and the following results were obtained.1. Out of 139 cultures tested, 95 were viable for this period in spite of the considerable change of temperature.2. All of 47 strains of Sacch. cerevisiae, S. cerevisiae var. ellipsoideus, S. marxianus and S. uvarum were preserved successfully.3. A large number of Schizosaccharomyces species lost viability within ca. three- to four-year period.4. No direct relationship between the longevity of these cultures stored by this method and their sporulating ability could be recognized, but in general, the cultures having sporulating ability seemed to be more stable than those which had lost this ability.
社団法人日本生物工学会の論文
1962-06-15