Streptomyces sp. の生産する Bacillus subtilis の熱死細胞溶解酵素 : 細菌細胞壁溶解酵素の研究(第3報)

概要

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A strain (L-13) of Streptomyces which produced lytic activity toward heat killed cells of Bacillus subtilis was isolated from soli. strain L-13 was able to produce the lytic activity by cultivation in medium containing albumin, casein, gelatin or fungal mycelium as an inducer as well as heat-killed cells of B. subtilis. The most favorable conditions thus obtained are as follows; The medium contained 0.4% of Aspergillus niger mycelium, 0.2% of K_2HPO_4 and 0.1% of MgSO_4・7H_2O (pH 6.0). Cultivation was carried out at 37℃ for 3 days with shaking. In the crude enzyme preparation produced by this strain under the above conditions, the activities of proteinase, chitinase, xylanase and β-1,3-glucanase were detected and cellulase and mannanase activities were not detected. To characterize the enzyme preparation, comparative column chroimatography including DEAE-cellulose, Biogel P-60 and disk electrophoresis were carried out. The results showed that lytic activity toward the heat killed cells of B. subtilis corresponded to the proteinase activity.
社団法人日本生物工学会の論文
1976-05-25