β-N-アセチルグルコサミニダーゼインヒビター生産菌によるインヒビターの生産条件とインヒビターの性質

概要

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Seven strains which produced β-N-acetylglucosaminidase inhibitors were obtained by using the Streptomyces enzyme. The inhibitors produced by these strains suppressed not only Streptomyces β-N-acetylglucosaminidase but also the enzyme from other sources, including plant and animal sources, to some extent. An Actinomycetes strain was selected from among the 7 strains as a potent inhibitor producer and used for subsequent experiments. Of the various carbon sources in Benett medium, N-acetylchitooligosaccharide and colloidal chitin markedly promoted production of the inhibitor. The culture broth was extracted with ethylacetate and n-butanol. Both the butanol and water layers exhibited β-N-acetylglucosaminidase inhibition. The inhibitor contained in the water layer was partially purified by successive column chromatography with active carbon and ion exchanger AG50W-X8. The purified inhibitor was active on various sources of β-N-acetylglucosaminidase such as Streptomyces sp., beans and abalone entrails, and kidney bean chitobiase, but had no effect on chitinases from Streptomyces antibioticus and Streptomyces griseus.
社団法人日本生物工学会の論文
1996-11-25