担子菌によるβ-N-アセチルグルコサミニダーゼの生産とその生理作用

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β-N-Acetylglucosaminidase activity in vegetative hypha, primordium, and fruit bodies of a strain of Pleurotus ostreatus was measured by quantitative and staining methods. Fruit bodies had the highest activity, followed by primordium and hypha. When the strain was cultivated at 22℃ for 3 weeks and then at 14℃ for 4 weeks as a static culture, it formed primordium at around 10 days after the temperature-shift, and gradually matured to fruit bodies afterward. Its growth reached the maximum at about 4-5 weeks afte inoculation and then decreased to form fruit bodies. Cellular GlcNAcase activity attained its maximum 1 week after inoculation and then gradually decreased until primordium formation, and increased again at fruiting. When the temperature-shift from 22℃ to 14℃, which was essential for fruiting, was not done, the increase in the activity of cellular GlcNAcase before formation of fruit bodies was not observed. This shows that the enzyme seems to act in the formation of fruit bodies. Growth of the strain and production of cellular and extracellular GlcNAcase at early growth stage were not affected by addition of N-acetylglucosamine or colloidsal chitin in the meidum. The strain, which was treated by temperature-shift before and after the increase of extracellular GlcNAcase activity, required a similar period for the formation of primordium and the maturity of fruit bodies.Cellular chitinase activity increased markedly at the period between formation of primordium and fruit bodies. The increase of the activity was not found when the strain was not treated by a temperature-shift from 22℃ to 14℃. It appears that chitinase as well as GlcNAcase plays some role in fruiting of the strain. When hypha at the period of fruiting, which had high chitinase and GlcNAcase activities, was incubated at 37℃ in buffer solution, N-acetylglucosamine was released from the hypha. Therefore, both chitinase and GlcNAcase in the mycelia appeared to participate in hydrolysis of cellular chitin to form GlcNAc which might be used as either a material for formation of fruit bodies or an energy source.
社団法人日本生物工学会の論文
1990-03-25

担子菌によるβ-N-アセチルグルコサミニダーゼの生産とその生理作用

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