細菌細胞壁溶解酵素の研究 : (第1報)火落菌溶解酵素生産菌株の分離と酵素生産条件の検討

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Many soil samples were screened for microorganisms able to produce enzymed lytic toward hiochic bacteria. The LA-7 strain of Streptomyces albus was isolated. A cell suspension of the LA-7 strain showed lytic action against both the gram-positive and gramnegative bacteria examined in this experiment to some extent.The LA-7 strain required the presence of cells of hiochic bacteria in order to produce larger amounts of the lytic enzyme. The experiment on the effects of nitrogen and carbon sources on the production of this enzyme showed that all of the inorganic and organic nitrogen compounds generally used as nitrogen sources had little effect; however, saccharose, xylose, and glycol chitin had some stimulative action. The enzyme was composed of protease and glycosidase but there was no amidase. The optimum pH value of this enzyme was 6.5. The pH stability was within a range of 4.0 to 7.0. About 90% of the activity was lost after at 75℃ for 30 min.
社団法人日本生物工学会の論文
1972-07-25