カビの菌糸体によるBacillus subtilis溶解酵素の誘導とその機構

概要

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Streptomyces sp. L-13 strain produced a lytic enzyme against Bacillus subtilis cells when grown on a medium containing mycelia of Aspergillus niger. The most effective fraction for the lytic enzyme production among several mycelial fractions prepared by means of Vibrongen cell mill disruption and centrifugation, was the crude cell wall fraction. However, even in a medium containing this fraction, no lytic activity was detected in the early period of growth, although chitinase and protease were found from the beginning. The lytic enzyme and β-1,3-glucanase were produced at a later period of growth.It was found that the reason for this was that the production of this lytic enzyme and β-1,3-glucanase was enhanced by N-acetylglucosamine and the soluble digest from the crude cell wall fraction with protease which was produced from the beginning of growth.The degradative activity of protease produced at a later stage of growth had unexpected lytic action against B. subtilis cells as well as A. niger mycelia.
社団法人日本生物工学会の論文
1980-03-25