Aspergillus nigerによるβ-N-アセチルグルコサミニダーゼの生産と酵素の局在

概要

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Some strains of fungi, yeasts and bacteria were tested for production of cellular β-N-acetylglucosaminidase. Most of the fungal strains tested, except for Mucor mucedo and Rhizopus javanicus, produced significant amounts of the enzyme. Yeast strains produced little or no enzyme, and a few bacteria produced it to some extent. Most of the enzyme from the strain of Aspergillus niger was intracellular. Localization of the enzyme in the mycelium of the strain was identified by staining the enzyme during mycelial development. Whole mycelia at early growth stage and the tips of mycelium had no enzyme activity. In a mycelial cell, the enzyme first appeared from the septa and then gradually spread all over the cell. The enzyme was also found in conidia formed by matured mycelia but not in conidiophores in which septa were not observed even at the late stage.
社団法人日本生物工学会の論文
1989-09-25