細菌細胞壁溶解酵素の研究 : (第2報)Streptomyces sp. LA-7菌の生産する火落菌溶解酵素の精製と諸性質について

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The lytic enzyme was purified from the culture broth of Streptomyces LA-7 as follows : fractionation with ammonium sulfate, gel filtration with Sephadex G-75,and chromatography on DEAE-cellulose. The purified enzyme showed a 32-fold increase in specific activity. The mode of action of the glycosidase was determined by analyzing the hydrolysates from the cell walls of Lactobacillus plantarum and chitin. This enzyme hydrolyzed the cell walls to N-acetylmuramic acid, N-acetylglucosamine, and a disaccharide which was identified as N-acetylmuramyl-N-acetylglucosamine by the Morgan-Elson reaction; chitin was hydrolyzed to N-acetylglucosamine and chitin dimer. The chitin dimer was not hydrolyzed at all. These results showed that this glycosidase is an endo-β-(1,4)-N-acetylglucosaminidase.
社団法人日本生物工学会の論文
1972-07-25