食品の核酸成分に関する研究 : (第18報)スイートコーンの核酸分解酵素系

概要

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Ribonuclease (RNase), phosphodiesterase (PDase), and phosphomonoesterase (PMase) were fractionated from edible portion of sweet corn and purified by the DEAE-Cellulose column technique. Their properties were examined by general methods. RNase from sweet corn had an optimum temperature of 60℃ and pH of 5.0. The ranges of optimum temperature and pH of PDase and PMase were 60℃ and pH 4.5-5.0,and 40℃-45℃ and pH 4.5-5.0 respectively. Four kinds of 5'-nucleotides, 5'-AMP, 5'-GMP, 5'-CMP, and 5'-UMP were obtained at pH 5.0 from the digestion of yeast RNA by sweet corn RNase. The PMase fraction decomposed ATP, 5'-AMP, and 3'-AMP. Furthermore, it was observed that 3'-AMP was decomposed more rapidly than 5'-AMP. The pH range of enzyme activities from sweet corn was different from that of shii-take (Lentinus edodes) and mushroom.
社団法人日本生物工学会の論文
1972-04-25