マッシュルームよりUDP-アセチルグルコサミンの分離

概要

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An acid soluble substance was isolated from the fruiting body of a mushroom Psalliota bisporus by column chromatography with Dowex 1×8 (formate type). This compound was purified in the form of its calcium salt. The calcium salt was degraded by treatment with diluted HCl and 5'-nucleotidase. It was found by paper and thin layer chromatography that the hydrolyzate consisted of uracil, uridine, uridine diphosphate, and N-acetyl-D-glucosamine. The original substance was identified as uridine diphospho-N-acetyl-D-glucosamine (UDPAG) by the direct comparison of its chromatographic behavior, and ultraviolet and infrared spectra with those of authentic UDPAG.It was roughly estimated that UDPAG formed about 15% of the total acid soluble and ultraviolet absorbing substances of the mushroom. This value corresponded to 0.42-0.69% of the dry weight of the mushroom.
社団法人日本生物工学会の論文
1971-08-25