担子菌による植物組織の崩壊 : (第1報)植物組織崩壊酵素生成菌株の検索

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As a result of a screening test for the production of macerating enzyme, it was found that only two out of 49 strains of Basidiomycetes, Fomitopsis cytisina (Berk) Bond et Sing and Irpex lacteus Fr. (Z-82), showed high activitiy for the maceration of potato-tuber as well as high activity for the dehulling of soybean.Certain culture conditions of these two strains for the high production of macerating enzyme were investigated.In the case of F. cytisina, the formation of the enzyme was satisfactory when 2-3% of pectin was added to 1 % sucrose rather than when only a carbohydrate such as sucrose was used as a carbon source in the medium. On the contrary, cellulose powder was the best carbon source for I. lacteus; it was better not to put any sugars such as sucrose with the cellulose powder. Glucose was rather repressive to the formation of macerating enzyme by the two strains.Although it was assumed that the enzyme taking part in the maceration of plant tissues was s sort of inducible enzyme, the enzyme was able to be produced sufficiently even in medium containing only sucrose as a carbon source, if the fermantation time was prolonged to a suitably long period. However, the higher the ratio of an inducible substance like pectin or cellulose powder to sucrose, the earlier a large amount of enzyme could be accumulated in the medium.Judging from the nature of the inducible substances used, the enzyme of F. cytisina was assumed to be a sort of pectinase and that of I. lacteus appeared to be a pectinase, too, in addition to a cellulolytic enzyme.
公益社団法人日本生物工学会の論文

担子菌による植物組織の崩壊 : (第1報)植物組織崩壊酵素生成菌株の検索

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