担子菌による植物組織の崩壊 : (第3報)粗酵素の分画

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Using enzyme preparations obtained from two strains of Basidiomycetes, Fomitopsis cytisina and Irpex lacteus, some comparisons of optimum pH, pH stability, and thermostability with respect to mecerating activity and certain polysaccharide decomposing activities such as cellulolytic, β-1,3-glucanase, xylanase, and polymethylgalacturonase activites, were performed. It all cases, the behavior of the pectolytic enzyme, which had a high activity for the liquefaction of pectin, and that of the macerating enzyme were in agreement with one another.Upon column chromatography of the enzyme preparations on several ion-exchange resins, it was observed that the pectolytic enzyme contained in these preparations was composed of many components differing from one another in chromatographic properties and that all of the fractions with a high activity for the maceration of plant tissues were always accompanied by a high activity for the liquefaction of pectin, but not vice versa.In connection with fractions that had a high pectolytic activity, some fractions showed only the pectolytic activity and no other polysaccharide decomposing activites were detected; another was unable to saccharify pectin and pectic acid, even though it was able to liquefy them. These fractions also showed a high macerating activity.Therefore, it is quite possibly a sort of endo-PG that was responsible for macerating plant tissues. The endo-PG liquefied pectin with a facility equal to or greater than pectic acid. However, some fractions were able to saccharify pectic acid to a much greater degree than pectin, so that it seemed that these fractions contained some exo-PG.
公益社団法人日本生物工学会の論文

担子菌による植物組織の崩壊 : (第3報)粗酵素の分画

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