不溶性サッカラーゼの調製とその性質

概要

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Saccharase was linked to DEAE-cellulose by means of ionic bond to make isoluble preparations. They containing 3.5-6.3 mg of saccharase/g of product and retaining 25-31 % of the activity of soluble enzyme. The optimum temperature of the isoluble saccharase was lower than that of the soluble sccharase, but the apparent activation energy was not changed. In the insoluble saccharase, it was also observed that the resistivity against mercuric acetate was increased slightly and that the thermal stability was decreased. But the drop in the enzymatic activity was scarcely observed after 15 weeks' storage at 4℃.The values of apparent Michaelis constant of the insoluble saccharase were calculated and shown to be dependent on the flow rate in column. On the other hand, the values of rate constant were nearly independent on the flow rate. A modified form of integrated Michaelis-Menten's equation was shown to be available to explain the action mechanism of these preparations.
社団法人日本生物工学会の論文
1971-07-25