Trichoderma Cellulaseの液内培養による生産と利用

概要

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Studies were conducted on the submerged culture of Trichoderma sp. for the production and utilization of cellulase. The cellulase productivity was determined by measuring the filter-paper degradating activity (FD) and the sodium cardoxy-methyl cellulose (CMC-Na) saccharifying activity (SP).A strain of Tricholderma viride WU-123CA was found to be highly efficient for the cellulase productivity, especially in the submerged culture process. The culture medium used in this experiment was prepared from an extract of wheat bran consisting also of KH_2PO_4 10,(NH_4)_2SO_4 3,NaNO_3 3,and MgSO_4・7H_2O 0.5 g/l. Cellulose powder (Toyo Roshi, 100-200 mesh) was found to be an important factor for inducing the cellulase formation. The culture conditions were also studide in detail, and the cellulase produced in the culture fluid was salted out quantitatively (about 0.5%) by the use of ammonium sulfate.This enzymatic preparation thus obtained also contained various kinds of enzymatic activities other than cellulase. It was used as an agent reacting on some vegetable foodstuffs and washed mycelia of microorganisms. The result indicates that the Tricoderma preparation obtained in this experiment seems to be effective for the quality improvement of foodstuffs. Also the enzymatic preparation promoted the digestion of cell walls to improve the efficiency of extracting useful substances contained in cells.
社団法人日本生物工学会の論文
1969-07-25