固定化酵素の調製時における酵素タンパクと有機溶媒との相互作用

概要

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An immobilized saccharsae was prepared by microencapsulation in polystyrene membrane through water-in-oil-in-water emulsions. The enzyme characteristics of microencapsulated saccharase differed from those of the native enzyme. The microcapsule had about 2.1% relative activity and the apparent Michaelis constant was about 3 times higher than that of native enzyme. The interaction between enzyme solution and organic solvent during emulsification was investigated to clarify the reasons for the low activity and change in conformation.Stirring was an important one mechanical factor effecting the interaction between enzyme solution and organic solvent. The relationship between stirring time and the residual activity was presented as a decreasing exponential functioin. It was found that benzene, ethyl acetate and cyclohexane were suitable organic soluvents.Saccharase treated with these solvents had about 38% residual activity and the absorbance of solutions in these solvents at 470 nm was 0.04〜0.10.
社団法人日本生物工学会の論文
1976-01-25