セロハン平板培養法によるAspergillus nigerの生育度と酵素活性およびクエン酸蓄積の関係

概要

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Aspergillus niger Yang no. 2 was cultivated on cellophane membranes placed on agar media, and the colonies formed were divided into areas every incubation day. Specific activities of enzymes related to citric acid accumulation were measured in each area for 2 to 8 days. Specific activitites of citrate synthase and isocitrate dehydrogenase (NADP) were low in the central area of the colony. With longer incubation, specific activity of isocitrate dehydrogenase (NADP) decreased as a whole. Isocitrate dehydrogenase (NAD) activity was not detected during the whole incubation period. The specific activity of aconitate hydratase was higher in the intermediate area of the colony than in the central and peripheral areas. Specific activities of isocitarate lyase and malate synthase in the central area of the colony were high, but that of pyruvate carboxylase was low.
公益社団法人日本生物工学会の論文
1985-01-25