半固体培養法によるクエン酸発酵におけるAspergillus nigerの酵素系の消長

概要

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The changes of enzyme activities during citric acid fermentation in solid culture were studied with a high citric acidproducing strain, Aspergillus niger Yang no. 2,cultivated in synthetic medium containing 2 g/l of gelatin and glass wool as carrier.With fermentation time, the specific activity ratio of fructose-bisphosphate aldolase to glucose-6-phosphate dehydrogenase increased. Of the TCA cycle enzmes, the specific activities of citrate synthetase and aconitate hydratase remained almost constant during the fermentation, while those of such enzymes as NADP-isocitrate dehydrogenase decreased remarkably during the period of citric acid accumulation. Activity of NAD-isocitrate dehydrogenase was not detected during the fermentation. Of the other enzymes related to the TCA cycle, the specific activity of isocitrate lyase increased and that of pyruvate carboxylase decreased during the period of citric acid accumulation.
社団法人日本生物工学会の論文
1984-05-25