光架橋性樹脂による酵素の固定(第2報) : パパインの固定化における諸要因

概要

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In the previous paper, photocrosslinkable oligomers were found to be useful materials for immobilization of papain. The photocrosslinking reaction could be occured very easily and under very mild conditions. In this paper, the effect of the immobilized matelials on the papain reactions was investigated. It became apparent that an initiator (benzoin ethyl ether) had little influence on the papain activity, on the other hand, photocrosslinking oligomers (polyethylene glycol dimthacrirate, and the like) had serious effect on that. In such cases, the papain activity was remarkably protected by modifying it's active site with mercury. And also, it was found that the reaction product (p-nitroaniline) was adosorbed by the polyethylene glycol dimethacrirate resin. The adosorption rate was approximately constant for each resin weight, and decreased with an increase in immobilized papain in resin. This immobilized papain showed the excellent stability for at least 20 batch reactions (presumably more than 100).
岡山理科大学の論文