水溶性高分子,ポリビニルアルコールによるパパインの固定化

概要

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Immobilization of papain by the use of water soluble polymer : polyvinyl alcohol (PVA) and the conditions for the preparation of immobilized papain were investigated. The results were obtained as follows : 1) To the 10% PVA water solution, papain was added, and the mixture was lyophilized, so that immobilized papain was easily obtained. 2) The PVA previously incubated in warm water resulted in a rise in the saponification rate, and also the PVA support prepared with the above PVA resulted in an increase in the thermal water stability. The PVA of 2000 degrees of polymerization was the best thermal water stability of the various PVA. 3) Immobilized papain prepeared PVA and papain in the proportion of 400mg to 20mg in a schale of 4.5 cm diameter showed some favorable properties. 4) This immobilized papain could withstand the continuous reaction for over 35 times, and showed high activity and stability.
岡山理科大学の論文