パパインの反応機構について : その反応動力学定数による推定

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Active-ionizing group and reaction mechanism of papain were investigated by using the following four substrates ; Casein, N-α-Benzoyl-DL-arginin-p-nitroanilide hydrochloride (BAPA), N-α-Benzoyl-L-arginin ethyl ester hydrochloride (BAEE), and N-Benzoyl-L-tyrosine ethyl ester (BTEE). The specificity of papain for various substrates was investigated on the basis of the reaction initial velocity and the kinetics constants were determined by reaction pH values. And then pK values were determined by using Dixon plot. From these results, in case BAPA and Casein were used, active-ionizing groups and reaction mechanism of papain were estimated as follows : A). Active-ionizing group of papain ; BAPA : Sulphydryl, Carboxyl. Casein : Sulphydryl, Imidazole. B). Reaction mechanism of papain ; refer to Fig.6.
岡山理科大学の論文