不完全酵母Trichosporon fermentans WU-C12による石油製品からのリパーゼの生産

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Production of extracellular lipase by Trichosporon fermentans WU-C12 with media containing petroleum products as carbon sources was studied. For lipase production, the most suitable nitrogen source and the most effective carbon sources were corn steep and the n-alkanes of C_9〜C_<12> respectively, with more than 20 U/ml of lipase being produced in 3d-cultivation. Of the petroleum products (n-paraffin, kerosene and gas oil), kerosene was the best carbon source for lipase production, with 32 U/ml of lipase being produced in 3 d-cultivation with a medium containing 3% (v/v) kerosene at 30℃. Addition of Tweenseries surfactants affected the lipase production, with Tween 85 being found most effective. When cultivated for 4 d in a medium containing 3% (v/v) kerosene and 0.5% (v/v) Tween 85,T. fermentans WU-C12 produced 60 U/ml of extracellular lipase.
公益社団法人日本生物工学会の論文
1992-05-25