界面活性剤の呈する酵素阻害作用 (II) : 界面活性剤とタカアミラーゼとの関係

概要

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Employing pure preparations of Taka-amylase A (TAA) and of Taka-amylase B (TAB) obtained by the methods, respectively, of Akabori and Okazaki, the actino of synthetic detergents upon Taka-amylases has been investigated. As detergents, SDS (sodium dodecyl sulfate), an anionic detergent, and OSVAN (alkyl dimethyl benzyl ammonium chloride), a cationic detergent, are used.The followings are some results obtained.1) TAB is less sensitive than TAA to the inhibiting action of these detergents.2) TAA is markedly inhibited by SDS when pH of the solution is below 4.0.3) When TAA solution is heated at pH 3 and 40℃ for 15 minutes in the presence of 1.2×0.001 M SDS (final conc.), the enzyme is completely inactivated, but TAB is not inactivated at least by the same treatment.4) TAA treated with the procedure mentioned above shows new ultraviolet absorption spectrum and the properties of completely denatured one.
社団法人日本生物工学会の論文
1961-04-15