繊維素分解菌に関する研究 (第4報) : 培養条件 (その2)

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Each component of the synthetic basal medium, McBeth's, has been examined to determine the factors affecting the formation of cellulase by the strains, T-s and T-4,isolated by the authors.The followings are the results obtained.1. The strains are able to utilize various sources of nitrogen. Ammonium slfate and peptone, respectively, as inorganic and organic sources, are more suitable than the others for producing cellulase in the medium, and their optimal concentrations in the medium are about 1%. Glycine and asparagine are also excellent sources of nitrogen.2. Cellulose (filter paper) and cellobiose are good carbon sources in producing cellulase. Filter paper is readily digested, while cotton and saw dust are done very slowly by the strains.Soluble starch and glucose are good sources for growth of the strains, though the production of cellulase becomes less. The less the concentration of cellulose is, the more of cellulose is digested when cellulose concentration in the medium is in the range from 0.05 to 4.0%.3. As to the effects of metalic salts in the medium, it is remarkable that molybdate exhibits stimulatory effect on the digestion of cellulose in the medium.
社団法人日本生物工学会の論文
1961-10-15