福島県で発生したインゲンマメつる枯病の病原ウイルス (クローバ葉脈黄化ウイルス) について

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A virus, designated NFU, isolated from bean plants (Phaseolus vulgaris cv. Kentucky Wonder) with severe vine necrosis (Turugare-byo) in Fukushima Prefecture was compared with a seed-borne isolate of bean yellow mosaic virus (BYMV-SB) from broad bean. When mechanically inoculated onto test plants, NFU caused severe necrotic symptoms on bean, broad bean and pea, whereas BYMV-SB caused mosaic symptoms. Isolate NFU was transmitted in a nonpersistent manner by Myzus persicae but not through seed. Virus particles and inclusion bodies showed typical potyvirus morphology when examined by electron microscopy. Analysis of a purified preparation of NFU by SDS-PAGE revealed two protein bands with relative molecular weights of 34 k and 37 k. In immunoelectron microscopy with some antisera against BYMV and clover yellow vein virus (CYVV), NFU was serologically more closely related to CYVV than BYMV. In enzyme linked immunosorbent assay (ELISA), agar gel diffusion test, immunoelectron microscopy, and Western blotting, NFU and BYMV-SB were sero1ogically related but antigenically different. Based on these results NFU was identified as a strain of CYVV. To find the weed-host plants of CYVV causing necrosis on bean, wild plants around bean fields were collected and assayed by ELISA and sap inoculation onto bean ; eight wild plants (Plantago asiatica, Veronica persica, Glechoma hederacea, Lamium amplexicaule, Perilla frutescens, Trfolium repens, T. pratense,Iris sp).) were infected with severe CYVV and thought to be important primary sources of inoculum for bean vine necrosis.
日本植物病理学会の論文
1992-04-25

福島県で発生したインゲンマメつる枯病の病原ウイルス (クローバ葉脈黄化ウイルス) について

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