Requirement of Negative Residues, Asp 95 and Asp 105, in S2 on Membrane Integration of a Voltage-dependent K^+ Channel, KAT1(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• Voltage-dependent K^+ channels consist of a voltagesensing region and a pore-forming region. Here we have identified the negative residues of the second transmembrane segment in the plant voltage-dependent K^+ channel, KAT1, which involves the function of voltage sensing. Point mutations at D95 and D105 but not D89 and D116 failed to complement the K^+ uptake deficient properties of the mutant yeast. In vitro translation and translocation experiments showed that the membrane integration of the third and fourth segments involving voltage sensor were impaired by the replacement of D95 or D105 by serine. These data show that both the residues play a crucial role in the membrane topogenesis of the voltage sensor in KAT1.

• 社団法人日本農芸化学会の論文
• 2003-04-23

著者

• SAKAGUCHI Masao

  Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University

• Uozumi N

  Nagoya Univ. Nagoya Jpn

• Sakaguchi Masao

  Department Of Molecular Biology Graduate School Of Medical Science Kyushu University

• Sakaguchi Masao

  Department Of Electronics And Control Engineering Nagano National College Of Technology

• UOZUMI Nobuyuki

  Graduate School of Bioagricutural Sciences, Nagoya University, Nagoya University Bioscience Center,

• Sakaguchi Masahiro

  Department Of Immunology And Infectious Disease Surveillance Center National Institute Of Infectious

• Sato Y

  Department Of Health Science Faculty Of Psychological And Physical Sciences Aichi Gakuin University

• SATO Yoko

  Graduate School of Bioagricultural Sciences, Nagoya University

• HOSOO Yoshihiro

  Graduate School of Bioagricultural Sciences, Nagoya University

• Hosoo Yoshihiro

  Graduate School Of Bioagricultural Sciences Nagoya University

• Sakaguchi M

  Osaka Univ. Pharmaceutical Sci. Osaka Jpn

• Sato Y

  Graduate School Of Bioagricultural Sciences Nagoya University

• Uozumi Nobuyuki

  Graduate School Of Bioagricultural Sciences

• Sato Yoko

  Graduate School Of Bioagricultural Sciences Nagoya University

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