Green Fluorescent Protein-labeled Recombinant Fluobody for Detecting the Picloram Herbicide(Organic Chemistry)
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概要
- 論文の詳細を見る
A green fluorescent protein-labeled fluobody was designed to develop a simple immunoassay method for detecting picloram herbicide in an environmental sample. The gfp gene was successfully inserted into the pSJF2 vector harboring the picloram-specific antibody fragment to yield pSJF2GFP. Picloram spiking in an environmental river sample could be indirectly detected by observing the fluorescence intensity value of the gfp-fluobody, exhibiting specific sensitivity to free picloram with an IC_<50> value of 50 ppb. Using the gfp-fluobody immunoassay avoids the enzyme-substrate reaction for calorimetric detection that is required in an enzyme-linked immunosorbent assay (ELISA).
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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KIM In
Department of Radiation Oncology, Seoul National University College of Medicine
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Shim Jae
Department Of Anesthesiology And Pain Medicine Yonsei University College Of Medicine
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Kim In
Department Of Animal Resource And Sciences Dankook University
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Shim J
Chonnam National Univ. Gwangju Kor
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Shim J
Division Of Applied Bioscience And Biotechnology Institute Of Agricultural Science And Technology Co
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LEE Hung
Department of Chemistryk, The Chinese University of Hong Kong
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Kim I
Department Of Environmental Science And Engineering Kwangju Institute Of Science And Technology (k-j
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Kim In
Department Of Chemical And Materials Engineering University Of Alberta
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Kim I
Kwangju Inst. Sci. And Technol. (k‐jist) Gwangju Kor
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SUH Yong
Department of Biological and Environmental Chemistry, Institute of Agricultural Science and Technolo
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YAU Kerrm
Department of Environmental Biology, University of Guelph
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HALL J.
Department of Environmental Biology, University of Guelph
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TREVORS Jack
Department of Environmental Biology, University of Guelph
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Suh Yong
Department Of Biological And Environmental Chemistry Institute Of Agricultural Science And Technolog
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Yau Kerrm
Department Of Environmental Biology University Of Guelph
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Trevors Jack
Department Of Environmental Biology University Of Guelph
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Lee Hung
Department Of Environmental Biology University Of Guelph
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Hall J.
Department Of Environmental Biology University Of Guelph
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Lee Hung
Department Of Chemistryk The Chinese University Of Hong Kong
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Shim Jae
Department Of Anesthesiology And Pain Medicine Anesthesia And Pain Research Institute Yonsei University College Of Medicine
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