Large-Scale Purification of Recombinant Monellin from Yeast
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概要
- 論文の詳細を見る
Recombinant sweet-tasting monellin was purified on a large scale (50 grams) from yeast strain AB110. The purification yield was 45%, and the purity was as high as 95%, as determined by HPLC gel filtration. Since monellin was proved to be a very basic protein (Cagan, R. : Science, 181,32-35,1973), teh basicity was utilized as an efficient purification method. Acid treatment for precipitating yeast proteins made proteins denatured except monellin.
- 社団法人日本生物工学会の論文
- 1996-08-25
著者
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KIM In
Department of Radiation Oncology, Seoul National University College of Medicine
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Kim In
Department Of Animal Resource And Sciences Dankook University
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Kim In
Department Of Chemical Engineering Chungnam National University
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Lim Kook
Biotechnology Department Lg Chemical Research Institute
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