Evaluation of Enzyme-Linked Immunosorbent Assays for the Detection of Cymbidium Mosaic Virus in Orchids
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概要
- 論文の詳細を見る
Indirect enzyme-linked immunosorbent assay(ELISA)and double antibody sandwich(DAS)ELISA using polyclonal antibodies(PAb), egg yolk immunoglobulin G(IgY), rabbit antisera(IgG)or monoclonal antibodies(MAb)alone, or any two in combination, were compared for the detection of Cymbidium mosaic virus(CyMV)in orchids. An indirect ELISA using IgG, IgY or MAb was able to detect 50-100 ng of purified virus and 2-8-fold dilution of infected leaf extract. Detection of purified CyMV by DAS-ELISA using IgY as the detecting antibody gave 4-16 times higher sensitivity than that using IgG on either MAb or PAb coated microplates. The DAS-ELISA using MAb as the coating antibody and IgY as the detecting antibody was shown to be the most sensitive, being able to detect 6.25 ng of purified virus and 1024-fold dilution of an infected leaf extract.
- 社団法人日本生物工学会の論文
- 1998-07-25
著者
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Ito K
Institute Of Molecular And Cell Biology And Oncology Research Institute National University Of Singa
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Ito K
Department Of Bio- And Geo-science Graduate School Of Science Osaka City University
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MINAMIURA Noshi
Faculty of Science, Osaka City University
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Iizuka Masaru
Faculty Of Science Osaka City University
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Iizuka Masaru
Department Of Bio- And Geo-science Graduate School Of Science Osaka City University
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ITO Kazuo
Faculty of Pharmacy, Meijo University
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Minamiura N
Department Of Bio- And Geo-science Graduate School Of Science Osaka City University
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Minamiura Noshi
Faculty Of Science Osaka City University
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Iizuka M
Department Of Bio- And Geo-science Graduate School Of Science Osaka City University
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VEJARATPIMOL RENU
Faculty of Science, Silpakorn University
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CHANNUNTAPIPAT CHOCKPISIT
Faculty of Science, Silpakorn University
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LIEWSAREE PITTAYA
Faculty of Industrial Technology, Silpakorn University
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REWNIM THANIT
Faculty of Science, Silpakorn University
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Rewnim Thanit
Faculty Of Science Silpakorn University
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Vejaratpimol Renu
Faculty Of Science Silpakorn University
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Liewsaree Pittaya
Faculty Of Industrial Technology Silpakorn University
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Ito Kazuo
Faculty Of Pharmacy Meijo University
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Channuntapipat Chockpisit
Faculty Of Science Silpakorn University
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