A Simple Method for Introducing Macromolecules into the Blastocoel of Living Starfish Embryos
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概要
- 論文の詳細を見る
We have devised a simple method for introducing macromolecules into the blastocoel of living starfish embryos. In this method, embryos are treated for 15 min with Ca^<2+> free sea water (CFSW) containing 0.1-5.0 mg/ml of enzymes or immunoglobulins. These molecules are considered to flow through the septate junction, the septa of which are made diffuse by deprivation of Ca^<2+> ions from the environmental sea water. Introduction of enzymes which affect the component molecules of the extracellular matrix (ECM) drastically deformed the larval morphology. Introduction of a monoclonal antibody recognizing an ECM component prevented the mesenchyme cells from stretching and migrating normally. These results show that our method can be used to study the function of various ECM components in embryonic development.
- 社団法人日本動物学会の論文
- 1995-10-15
著者
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Kaneko Hiroyuki
Department of Genetic Resources II, National Institute of Agrobiological Resources, Tsukuba, Ibaraki
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Dan-Sohkawa Marina
Department of Biology, Faculty of Science, Osaka City University
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Iizuka Masaru
Department Of Bio- And Geo-science Graduate School Of Science Osaka City University
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Iizuka Masaru
Department Of Biology Faculty Of Science Osaka City University
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Dan-sohkawa Marina
Department Of Biology Faculty Of Science Osaka City University
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Okushima Yasuo
Department of Biology, Faculty of Science, Osaka City University
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Okushima Yasuo
Department Of Biology Faculty Of Science Osaka City University
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Kaneko Hiroyuki
Department Of Animal Production Kyushu National Agricultural Experiment Station
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Kaneko Hiroyuki
Department Of Biology Faculty Of Science Osaka City University
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