Isolation of Molecular Markers Linked to the Cry Locus Conferring Resistance to Cucumber mosaic cucumovirus Infection in Cowpea
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概要
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We previously demonstrated that cowpea[Vigna unguiculata(L.)Walp.]cultivar Kurodane-Sanjaku contains the Cry gene, which confers resistance against Cucumber mosaic cucumovirus infection. In this paper, randomly amplified polymorphic DNA(RAPD)analysis was carried out to tag the Cry locus. Bulked segregant analysis for RAPD resulted in many polymorphisms in amplified DNA patterns. Candidates were further screened using parental and/or F_2 cowpea DNAs. As a result, we obtained three RAPD markers, D13/E14-350, WA3-850 and OPE3-500, flanking the Cry locus. In addition, we amplified cowpea sequences coding for the putative nucleotide-binding site(NBS). Degenerate primers based on NBS sequences of tobacco N and Arabidopsis RPS2 disease resistance genes were used for polymerase chain reaction, and resultant products were cloned and sequenced. Among eight independent clones, cowpea resistance gene analog(CRGA)5 showed a distinct polymorphism when used as a probe for restriction fragment length polymorphism analysis against the susceptible cowpea cultivar PI 189375 and a near-isogenic line for the Cry. Linkage analyses of these molecular markers showed that genetic distances of CRGA5, D13/E14-350, WA3-850 and OPE3-500 to the Cry locus were 0.7, 5.2, 11.5 and 24.5cM, respectively.
- 日本植物病理学会の論文
- 2000-08-25
著者
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Karasawa Akira
協和醗酵工業
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Karasawa A
Drug Development Research Laboratories Pharmaceutical Research Institute Kyowa Kogyo Co. Ltd.
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Karasawa Akira
Pharmaceuticals Research Laboratory Kyowa Hakko Kogyo Co. Ltd.
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Karasawa Akira
Laboratory Of Plant Pathology Department Of Applied Life Science Graduate School Of Agricultural Sci
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EHARA Yoshio
Laboratory of Plant Pathology, Faculty of Agriculture, Tohoku University
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Takahashi Hideki
Laboratory of Plant Pathology, Tokyo University of Agriculture & Technology
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Hase S
Department Of Life Science Graduate School Of Agricultural Science Tohoku University
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Ehara Y
Tohoku Univ. Sendai Jpn
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Ehara Yoshio
Laboratory Of Plant Pathology Faculty Of Agriculture Tohoku University
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CHIDA Yutaka
Laboratory of Plant Pathology, Department of Applied Life Science, Graduate School of Agricultural S
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OKAZAKI Kazuyuki
Laboratory of Plant Pathology, Department of Applied Life Science, Graduate School of Agricultural S
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AKASHI Kayoko
Laboratory of Plant Pathology, Department of Applied Life Science, Graduate School of Agricultural S
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NAKAZAWANASU Yoshiko
Laboratory of Plant Pathology, Department of Applied Life Science, Graduate School of Agricultural S
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HASE Shuu
Laboratory of Plant Pathology, Department of Applied Life Science, Graduate School of Agricultural S
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Karasawa Akira
Pharmaceutical Research Institute Kyowa Hakko Kogyo Co. Ltd.
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Hase Shuu
Laboratory Of Plant Pathology Graduate School Of Agricultural Sciences Tohoku University
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Chida Yutaka
Laboratory Of Plant Pathology Department Of Applied Life Science Graduate School Of Agricultural Sci
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Akashi Kayoko
Laboratory Of Plant Pathology Department Of Applied Life Science Graduate School Of Agricultural Sci
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Okazaki Kazuyuki
Laboratory Of Plant Pathology Department Of Applied Life Science Graduate School Of Agricultural Sci
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Nakazawanasu Yoshiko
Laboratory Of Plant Pathology Department Of Applied Life Science Graduate School Of Agricultural Sci
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Takahashi Hideki
Laboratory Of Plant Pathology Department Of Applied Life Science Graduate School Of Agricultural Sci
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Takahashi Hideki
Laboratory Of Applied Microbiology Faculty Of Agriculture Yamagata University
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