Observation of Microorganisms Colonies Using a Scanning-Laser-Beam pH-Sensing Microscope

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概要

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• The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results.

• 社団法人日本生物工学会の論文
• 1995-02-25

著者

• IWASAKI Hiroshi

  The Institute of Scient4fic and Industrial Research, Osaka University

• YOSHINOBU Tatsuo

  The Institute of Scientific and Industrial Research, Osaka University

• Yoshinobu T

  Osaka Univ. Osaka Jpn

• Yoshinobu Tatsuo

  The Institute Of Scientific And Industrial Research Osaka University

• Yoshinobu Tatsuo

  Department Of Electrical Engineering Kyoto University:(present Address) The Institute Of Scientific

• NAKAO Motoi

  Horiba Ltd.

• NAKAO MOTOI

  The Institute of Scientific and Industrial Research, Osaka University

• INOUE SHINJI

  The Institute of Scientific and Industrial Research, Osaka University

• OISHI RYU

  Nakano Central Research Institute, Nakano Vinegar Co. Ltd.

• Oishi Ryu

  Nakano Central Research Institute Nakano Vinegar Co. Ltd.

• Inoue S

  Yokohama National Univ. Yokohama

• Inoue Shinji

  Biomass Research Group Institute For Energy Utilization National Institute Of Advanced Industrial Sc

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