Strategy and Design of Novel Reagents for the Fluorometric Analysis of Biomolecules
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概要
- 論文の詳細を見る
This article covers molecular designs to develop several new fluorometric reagents and their applications to increase the sensitivities up to the picomole level using HPLC for the measurement of biomolecules. The methods were designed to demonstrate the physiological activities, for example (1) N-(9-acridinyl)maleimide (NAM) for the measurement of SH, -S-S-, and sulfite such as cysteine, (2) diphenyl-1-pyrenylphosphine (DPPP) for the hydroperoxides in lipids, serum, tissues, and foodstuffs, (3) 9-bromomethylacridine (9-BrMA), (4) 2-(anthracene-2,3-dicarboxylimide)ethyltrifluoromethane sulfonate (AE-OTf) for carboxylic acids, and (5) The chiral fluorometric labelling reagent (S)-(+)-2-tert-butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid (TBMB) to identify the chiralities of amino acids, sugars, and mono- and diacylglycerols.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
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OHRUI Hiroshi
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
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Ohrui Hiroshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Meguro Hiroshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Meguro Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
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