ステリセルプロセッサーの末梢血幹細胞分離及び凍結保存への適用

概要

論文の詳細を見る
Peripheral blood stem cell(PBSC)transplantation has been widely accepted as a treatment for hematologic and other malignancies. PBSCs are harvested by leukapheresis, but the harvest is usually contaminated with red cells, platelets and granulocytes. Post-leukapheresis processing is required to purify mononucler cells(MNC)which contain PBSCs from other contaminating cells to cryoproserve stem cells with good recovery. However, the procedure is time-consuming, complex, and has a hazard of contamination.The SteriCell processor is designed to purify MNC by density gradient separation using Ficoll-Paque and wash them with saline automatically in a closed system. In the automated process, the mean recovery of MNC and hematopietic progenitor cells from the leukapheresis product was 77 and 75%, respectively. Recoveries of MNC, CFU-GM, and BFU-E after cryopreservation were 89.5%, 73.7%, and 45.4%, respectively. The SteriCell processor should be useful to purify MNC from the leukaphereresis product in a short time in a closed system.
日本医療機器学会の論文
1993-02-01