血液細胞の凍結保存用バッグの開発と評価

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Cryopreservation is a method used to store blood cells for a long period without loss of viability and functions. However, the method is rather limited because the procedures of freezing and thawing, and removal of cryoprotectants are complicated. In addition, it requires an expensive programming freezer and special freezing bags which do not break at low temperature. In this study, we developed a new freezing bag (KFB : Kawasumi Freezing Bag) which is made of laminated plastic composed of fluoride and polyolefin. The tensile strength and elongation percentage at break and gas permeability of laminated plastic sheet were examined in accord with Japan Industrial Standard. The tensile strength at -80℃ and the gas permeability of laminated plastic sheet were almost equal to those of a polyvinyl chloride (PVC) sheet. However, the elongation percentage of laminated plastic sheet was twice as much as the PVC sheet at -80℃. Platelets, red cells and peripheral blood stem cells were frozen in the newly developed freezing bags with their standard procedures. Platelets and peripheral blood stem cells were stored in liquid nitrogen, and red cells were stored at -80℃. There were no significant differences in the numerical recovery and the overall functions between cells frozen in the new freezing bag and the conventional bags (PVC or ethylene vinyl acetate). The freezing bags did not break in liquid nitrogen. These results suggest that the freezing bag developed in this study is useful for cryopreservation of blood cells in liquid nitrogen.
日本医療機器学会の論文
1995-01-01

血液細胞の凍結保存用バッグの開発と評価

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