2. 東京臍帯血バンクにおける臍帯血プロセッシングと凍結保存(平成10年度第44回低温生物工学会研究報告)

概要

論文の詳細を見る
Cryopreservation of human placental/umbilical cord blood (PCB) is required for PCB banking. We collected and separated PCB following the New York Blood Center's protocol and cryopreserved them in liquid nitrogen (LN_2). Red cells were sedimented by addition of 1% hydroxyethyl starch with gentle centrifugation. Mean recovery of nucleated cells (NC) and colony-forming units (CPU) was 82.9% and 90.4%, respectively (n=25). Volume of the NC suspension was further reduced to 20 ml by centrifugation and dimethyl sulfoxide and Dextran40 solution were added slowly to a final concentration of 10% and 1%, respectively. Those units were cryopreserved in a -80℃ mechanical freezer slowly, and transferred to LN_2 storage tank. Thawing of PCB was done rapidly, and the frozen cells were washed with a solution containing 10% Dextran 40 and 5% human serum albumin. NC and CPU recovery was 88.8 and 81.2%, respectively. Thus, the HES/slow cooling method has proved to be quite useful for cell processing of cord blood. In the Tokyo Cord Blood Bank, we separate, concentrate and cryopreserve cord blood nucleated cells following this method, and the method seems to have become the standard method for other local cord blood banks in Japan.
低温生物工学会の論文
1998-12-28