15. ヒト臍帯血巨核球コロニー形成細胞CFU-Megの高凍結感受性(平成11年度第45回低温生物工学会研究報告)

概要

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We found that the colony-forming units- megakaryocyte (CFU-Meg) in human umbilical/ placental cord blood (PCB) are sensitive to freezing injuries during cryopreservation. Mononuclear cells isolated from PCB were cryopreserved by the standard method used for the PCB banking. The recovery of CFU-Meg were less than other colony-forming cells. The cells were exposed to various osmotic stresses, cooled at different cooling rates, plunged into liquid nitrogen from different temperatures. Under these stresses, CFU-Meg were more sensitive than other colony forming cells. These results indicate that CFU-Meg are sensitive to freezing injury. This may be one of the reasons that platelet engraftment is slow in PCB transplantation.
低温生物工学会の論文
1999-12-30